鸽源新城疫病毒全基因组序列分析及致病性研究

doi:10.11843/j.issn.0366-6964.2014.02.013

Abstract

Abstract:

The genome and pathogenicity of pigeon NDV isolate were characterized for better understanding the evolution of NDV.A strain of Newcastle disease virus，named Pigeon/China/SDLC/2011，was isolated and identified from diseased pigeon flock.The full-length genome of Pigeon/China/SDLC/2011 was sequenced and compared with reference strains.Besides，the pathogenicity of Pigeon/China/SDLC/2011 was evaluated in pigeons and SPF chickens.Sequence analysis showed that the complete genome of Pigeon/China/SDLC/2011 contained 15 192 nucleotides(nt).The cleavage site of the fusion protein was¹¹²RRQKRF¹¹⁷，a feature generally associated with virulent NDV strains.Phylogenetic analysis，based on the genomic sequences and sequences of the fusion gene，revealed that Pigeon/China/SDLC/2011 should be classified as genotype Ⅵ NDV.Mutations were observed in the six structural proteins of Pigeon/China/SDLC/2011 compared with reference NDV strains of genotype Ⅶ.More mutations were localized in the P and HN protein than the other proteins and the amino acid sequence identities of P and HN proteins between Pigeon/China/SDLC/2011 and genotype Ⅶ NDVs was 84.6%-84.8%，90.0%-91.1%，respectively.In the challenge test，70%-80% of pigeons inoculated with Pigeon/China/SDLC/2011 showed a series of nervous disorders：bilateral or unilateral locomotor disturbances of wings or legs，torticollis，and watery green diarrhea and died in 6-14 days.However，chickens remained clinically normal throughout the study.Viruses were detected more frequently in the swabs of pigeons than chickens.The results showed that marked differences in molecular characterization and pathogenicity in chickens were recorded between Pigeon/China/SDLC/2011 and the virulent NDVs of genotype Ⅶ.Although Pigeon/China/SDLC/2011 was nopathogenic for chickens，some measures should be taken to prevent virus transmission between pigeions and chickens as viruses were detected in swabs of infected chickens.

CLC Number:

HU Bei-xia，YANG Shao-hua，ZHANG Hui-juan，YI Hui，ZHANG Wei，WANG Li-li，XU Chuan-tian，ZHANG Lin,HUANG Qing-hua，ZHANG Xiu-mei. Complete Genome Sequence Analysis and Pathogenicity of Newcastle Disease Virus Isolated from Pigeons[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 255-261.

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Complete Genome Sequence Analysis and Pathogenicity of Newcastle Disease Virus Isolated from Pigeons

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